PoplarV1, Main, Exploration, bibRecord, 001801

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts.

Identifieur interne : 001801 ( Main/Exploration ); précédent : 001800; suivant : 001802

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts.

Auteurs : Jung-Hun Kim [Corée du Sud] ; Chonglong Wang [Corée du Sud] ; Hui-Jung Jang [Corée du Sud] ; Myeong-Seok Cha [Corée du Sud] ; Ju-Eon Park [Corée du Sud] ; Seon-Yeong Jo [Corée du Sud] ; Eui-Sung Choi [Corée du Sud] ; Seon-Won Kim [Corée du Sud]

Source :

Microbial cell factories [ 1475-2859 ] ; 2016.

RBID : pubmed:28010736

Descripteurs français

KwdFr :
- Acide mévalonique (métabolisme), Alkyl et aryl transferases (génétique), Alkyl et aryl transferases (métabolisme), Butadiènes (métabolisme), Escherichia coli (génétique), Escherichia coli (métabolisme), Fermentation (MeSH), Hémiterpènes (métabolisme), Pentanes (métabolisme), Populus (enzymologie), Populus (génétique).
MESH :
- enzymologie : Populus.
- génétique : Alkyl et aryl transferases, Escherichia coli, Populus.
- métabolisme : Acide mévalonique, Alkyl et aryl transferases, Butadiènes, Escherichia coli, Hémiterpènes, Pentanes.
- Fermentation.

English descriptors

KwdEn :
- Alkyl and Aryl Transferases (genetics), Alkyl and Aryl Transferases (metabolism), Butadienes (metabolism), Escherichia coli (genetics), Escherichia coli (metabolism), Fermentation (MeSH), Hemiterpenes (metabolism), Mevalonic Acid (metabolism), Pentanes (metabolism), Populus (enzymology), Populus (genetics).
MESH :
- chemical , genetics : Alkyl and Aryl Transferases.
- chemical , metabolism : Alkyl and Aryl Transferases, Butadienes, Hemiterpenes, Mevalonic Acid, Pentanes.
- enzymology : Populus.
- genetics : Escherichia coli, Populus.
- metabolism : Escherichia coli.
- Fermentation.

Abstract

BACKGROUND

Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives.

RESULTS

In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80 mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337 mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (P

CONCLUSIONS

We achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation.

DOI: 10.1186/s12934-016-0612-6
PubMed: 28010736
PubMed Central: PMC5180398

Affiliations:

Corée du Sud

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Wang, Chonglong" sort="Wang, Chonglong" uniqKey="Wang C" first="Chonglong" last="Wang">Chonglong Wang</name>
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<series><title level="j">Microbial cell factories</title>
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<term>Alkyl and Aryl Transferases (metabolism)</term>
<term>Butadienes (metabolism)</term>
<term>Escherichia coli (genetics)</term>
<term>Escherichia coli (metabolism)</term>
<term>Fermentation (MeSH)</term>
<term>Hemiterpenes (metabolism)</term>
<term>Mevalonic Acid (metabolism)</term>
<term>Pentanes (metabolism)</term>
<term>Populus (enzymology)</term>
<term>Populus (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Acide mévalonique (métabolisme)</term>
<term>Alkyl et aryl transferases (génétique)</term>
<term>Alkyl et aryl transferases (métabolisme)</term>
<term>Butadiènes (métabolisme)</term>
<term>Escherichia coli (génétique)</term>
<term>Escherichia coli (métabolisme)</term>
<term>Fermentation (MeSH)</term>
<term>Hémiterpènes (métabolisme)</term>
<term>Pentanes (métabolisme)</term>
<term>Populus (enzymologie)</term>
<term>Populus (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Alkyl and Aryl Transferases</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alkyl and Aryl Transferases</term>
<term>Butadienes</term>
<term>Hemiterpenes</term>
<term>Mevalonic Acid</term>
<term>Pentanes</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Alkyl et aryl transferases</term>
<term>Escherichia coli</term>
<term>Populus</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acide mévalonique</term>
<term>Alkyl et aryl transferases</term>
<term>Butadiènes</term>
<term>Escherichia coli</term>
<term>Hémiterpènes</term>
<term>Pentanes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Fermentation</term>
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<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80 mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337 mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (P</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>We achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28010736</PMID>
<DateCompleted><Year>2017</Year>
<Month>03</Month>
<Day>28</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1475-2859</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>15</Volume>
<Issue>1</Issue>
<PubDate><Year>2016</Year>
<Month>Dec</Month>
<Day>23</Day>
</PubDate>
</JournalIssue>
<Title>Microbial cell factories</Title>
<ISOAbbreviation>Microb Cell Fact</ISOAbbreviation>
</Journal>
<ArticleTitle>Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts.</ArticleTitle>
<Pagination><MedlinePgn>214</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s12934-016-0612-6</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Isoprene, a volatile C5 hydrocarbon, is an important platform chemical used in the manufacturing of synthetic rubber for tires and various other applications, such as elastomers and adhesives.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">In this study, Escherichia coli MG1655 harboring Populus trichocarpa isoprene synthase (PtispS) and the exogenous mevalonate (MVA) pathway produced 80 mg/L isoprene. Codon optimization and optimal expression of the ispS gene via adjustment of the RBS strength and inducer concentration increased isoprene production to 199 and 337 mg/L, respectively. To augment expression of MVA pathway genes, the MVA pathway was cloned on a high-copy plasmid (pBR322 origin) with a strong promoter (P<sub>trc</sub>
), which resulted in an additional increase in isoprene production up to 956 mg/L. To reduce the formation of byproducts derived from acetyl-CoA (an initial substrate of the MVA pathway), nine relevant genes were deleted to generate the E. coli AceCo strain (E. coli MG1655 ΔackA-pta, poxB, ldhA, dld, adhE, pps, and atoDA). The AceCo strain harboring the ispS gene and MVA pathway showed enhanced isoprene production of 1832 mg/L in flask culture with reduced accumulation of byproducts.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We achieved a 23-fold increase in isoprene production by codon optimization of PtispS, augmentation of the MVA pathway, and deletion of genes involved in byproduct formation.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Jung-Hun</ForeName>
<Initials>JH</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Research Center for Industrial Chemical Biotechnology, KRICT, Ulsan, 44468, South Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Chonglong</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jang</LastName>
<ForeName>Hui-Jung</ForeName>
<Initials>HJ</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Life Science Research Institute, Daewoong Pharmaceutical Corporation, Yongin, South Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Cha</LastName>
<ForeName>Myeong-Seok</ForeName>
<Initials>MS</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Park</LastName>
<ForeName>Ju-Eon</ForeName>
<Initials>JE</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Jo</LastName>
<ForeName>Seon-Yeong</ForeName>
<Initials>SY</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Choi</LastName>
<ForeName>Eui-Sung</ForeName>
<Initials>ES</Initials>
<AffiliationInfo><Affiliation>Industrial Biotechnology Research Center, KRIBB, Daejeon, 305-806, South Korea. choi4162@kribb.re.kr.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Kim</LastName>
<ForeName>Seon-Won</ForeName>
<Initials>SW</Initials>
<AffiliationInfo><Affiliation>Division of Applied Life Science (BK21 Plus), PMBBRC, Institute of Agricultural and Life Science, Gyeongsang National University, Jinju, 52828, South Korea. swkim@gnu.ac.kr.</Affiliation>
</AffiliationInfo>
</Author>
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<Language>eng</Language>
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<Month>12</Month>
<Day>23</Day>
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<MedlineTA>Microb Cell Fact</MedlineTA>
<NlmUniqueID>101139812</NlmUniqueID>
<ISSNLinking>1475-2859</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D002070">Butadienes</NameOfSubstance>
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<NameOfSubstance UI="C005059">isoprene</NameOfSubstance>
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<Chemical><RegistryNumber>EC 2.5.-</RegistryNumber>
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<Chemical><RegistryNumber>EC 2.5.1.-</RegistryNumber>
<NameOfSubstance UI="C093854">isoprene synthase</NameOfSubstance>
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<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Bioisoprene</Keyword>
<Keyword MajorTopicYN="Y">Carbon utilization</Keyword>
<Keyword MajorTopicYN="Y">Escherichia coli</Keyword>
<Keyword MajorTopicYN="Y">Isoprene synthase</Keyword>
<Keyword MajorTopicYN="Y">Mevalonate pathway</Keyword>
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<Day>13</Day>
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<Month>12</Month>
<Day>04</Day>
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<Day>25</Day>
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<Day>30</Day>
<Hour>6</Hour>
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<ArticleId IdType="doi">10.1186/s12934-016-0612-6</ArticleId>
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<ArticleId IdType="pmc">PMC5180398</ArticleId>
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<affiliations><list><country><li>Corée du Sud</li>
</country>
</list>
<tree><country name="Corée du Sud"><noRegion><name sortKey="Kim, Jung Hun" sort="Kim, Jung Hun" uniqKey="Kim J" first="Jung-Hun" last="Kim">Jung-Hun Kim</name>
</noRegion>
<name sortKey="Cha, Myeong Seok" sort="Cha, Myeong Seok" uniqKey="Cha M" first="Myeong-Seok" last="Cha">Myeong-Seok Cha</name>
<name sortKey="Choi, Eui Sung" sort="Choi, Eui Sung" uniqKey="Choi E" first="Eui-Sung" last="Choi">Eui-Sung Choi</name>
<name sortKey="Jang, Hui Jung" sort="Jang, Hui Jung" uniqKey="Jang H" first="Hui-Jung" last="Jang">Hui-Jung Jang</name>
<name sortKey="Jang, Hui Jung" sort="Jang, Hui Jung" uniqKey="Jang H" first="Hui-Jung" last="Jang">Hui-Jung Jang</name>
<name sortKey="Jo, Seon Yeong" sort="Jo, Seon Yeong" uniqKey="Jo S" first="Seon-Yeong" last="Jo">Seon-Yeong Jo</name>
<name sortKey="Kim, Jung Hun" sort="Kim, Jung Hun" uniqKey="Kim J" first="Jung-Hun" last="Kim">Jung-Hun Kim</name>
<name sortKey="Kim, Seon Won" sort="Kim, Seon Won" uniqKey="Kim S" first="Seon-Won" last="Kim">Seon-Won Kim</name>
<name sortKey="Park, Ju Eon" sort="Park, Ju Eon" uniqKey="Park J" first="Ju-Eon" last="Park">Ju-Eon Park</name>
<name sortKey="Wang, Chonglong" sort="Wang, Chonglong" uniqKey="Wang C" first="Chonglong" last="Wang">Chonglong Wang</name>
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Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts.

Isoprene production by Escherichia coli through the exogenous mevalonate pathway with reduced formation of fermentation byproducts.

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